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Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit

SKU:
HUDL00023
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O15438
Sensitivity:
0.105ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ABC31,MLP2,MOATD,MRP3,cMOAT2,Canalicular Multispecific Organic Anion Transporter 2,Multi-specific organic anion transporter D,Multidrug resistance-associated protein 3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit

The Human ATP binding cassette transporter C3 (ABCC3) ELISA Kit is specially designed for the precise measurement of ABCC3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and accuracy, delivering consistent and dependable results, which makes it an invaluable tool for various research applications.ABCC3, also known as multidrug resistance-associated protein 3 (MRP3), is a key transporter protein involved in drug resistance and detoxification processes.

Its dysregulation has been linked to various diseases such as cancer, inflammatory disorders, and liver diseases, highlighting its significance as a potential biomarker for studying these conditions and developing therapeutic interventions.With the Human ABCC3 ELISA Kit, researchers can gain valuable insights into the role of ABCC3 in disease progression and treatment response, paving the way for advancements in personalized medicine and drug development strategies.

Product Name:Human ATP Binding Cassette Transporter C3 (ABCC3) ELISA Kit
Product Code:HUDL00023
Size:96 Assays
Target:ABCC3
Synonyms:ABC31, MLP2, MOATD, MRP3, cMOAT2, Canalicular Multispecific Organic Anion Transporter 2, Multi-specific organic anion transporter D, Multidrug resistance-associated protein 3
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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