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Human Atherosclerosis Array (20 targets) (SARB0024)

SKU:
SARB0024
Product Type:
Protein Array
Analytes:
Eotaxin-1 (CCL11)
GCSF
GDNF
GM-CSF
ICAM-1 (CD54)
IFN-gamma
IL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2)
IL-1 R1
IL-1 R2
MCP-1 (CCL2)
M-CSF
TGF beta 1
TGF beta 2
TGF beta 3
TNF alpha
TNF beta (TNFSF1B)
MIP-3 alpha (CCL20)
PDGF-BB
RANTES (CCL5)
Reactivity:
Human
Applications:
Multiplex Array
$803 - $1,559

Description

system_update_altDatasheet

Human Atherosclerosis Array (20 targets) (SARB0024)

The Human Atherosclerosis Array (SARB0024) from Assay Genie is a comprehensive research tool designed to analyze 20 key targets associated with atherosclerosis, a chronic inflammatory disease that affects the arteries. This array allows researchers to simultaneously measure the levels of various proteins involved in the pathogenesis of atherosclerosis, including cytokines, chemokines, and growth factors.With this array, researchers can gain valuable insights into the molecular mechanisms underlying atherosclerosis and identify potential therapeutic targets for the treatment of this disease.

The assay is easy to use, requires a minimal sample volume, and provides accurate and reproducible results, making it an essential tool for studies in cardiovascular research and drug development.By utilizing the Human Atherosclerosis Array from Assay Genie, researchers can accelerate their research efforts and make significant advancements in the understanding and management of atherosclerosis. Order this array today and take a step closer to unraveling the complexities of this prevalent and life-threatening condition.

Product Sku:SARB0024
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:CCL11, CCL2, CCL20, CCL5, CSF1, CSF2, CSF3, GDNF, ICAM1, IFNG|IL1A, IL1B, IL1R1, IL1R2, LTA, PDGFB, TGFB1, TGFB2, TGFB3, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:MAPK Signaling / NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
ICAM-1 (CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-1 R1
  • Gene Symbol: IL1R1
  • Gene ID: 3554
  • Accession #: P14778
IL-1 R2
  • Gene Symbol: IL1R2
  • Gene ID: 7850
  • Accession #: P27930
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
TGF beta 2
  • Gene Symbol: TGFB2
  • Gene ID: 7042
  • Accession #: P61812
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: 6364
  • Accession #: P78556
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Atherosclerosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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