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Human ATF4 / Activating Transcription Factor 4 ELISA Kit

SKU:
HUFI02240
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P18848
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
ATF-4, TAXREB67, TXREB, Activating transcription factor 4, activating transcription factor 4, tax-responsive enhancer element B67, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB-2DNA-binding protein TAXREB6
Reactivity:
Human
Research Area:
Cell Biology
€599
Frequently bought together:

Description

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Human ATF4 / Activating Transcription Factor 4 ELISA

ATF4 (Activating Transcription Factor 4) is a transcription factor that is specifically expressed in activated T cells. ATF4 regulates the expression of a number of genes involved in cell activation, including IL-2 and TNF-alpha (tumor necrosis factor-alpha). Diseases associated with ATF4 include Coffin-Lowry Syndrome and Leukoencephalopathy With Vanishing White Matter. Pathways associated with AFT4 include CREB Pathway and ERK Signaling. An important paralog of ATF4 is ATF5.

Product Name:Human ATF4 / Activating Transcription Factor 4 ELISA Kit
Product Code:HUFI02240
Size:96 Assays
Alias:ATF-4, TAXREB67, TXREB, Activating transcription factor 4, activating transcription factor 4, tax-responsive enhancer element B67, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB-2DNA-binding protein TAXREB67, cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2, TaxREB67, TAXREB67CREB2cAMP response element-binding protein 2, Tax-responsive enhancer element-binding protein 67, TXREB
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human ATF-4 concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human ATF-4 and the recovery rates were calculated by comparing the measured value to the expected amount of Human ATF-4 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)85-9591
EDTA plasma(n=5)86-10392
UFH plasma(n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ATF-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)86-101%85-102%87-98%
EDTA plasma(n=5)89-94%86-98%85-99%
UFH plasma(n=5)83-93%80-93%82-98%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP18848
UniProt Protein Function:ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described.
UniProt Protein Details:Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus

Molecular Function:protein C-terminus binding; leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: amino acid metabolic process; transcription from RNA polymerase II promoter; positive regulation of apoptosis; unfolded protein response; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of transcription from RNA polymerase I promoter; gluconeogenesis; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway

NCBI Summary:This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]
UniProt Code:P18848
NCBI GenInfo Identifier:116241262
NCBI Gene ID:468
NCBI Accession:P18848.3
UniProt Secondary Accession:P18848,Q9UH31,
UniProt Related Accession:P18848
Molecular Weight:Calculated Molecular Weight: 38kDaObserved Molecular Weight: 38kDa
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-4
NCBI Synonym Full Names:activating transcription factor 4
NCBI Official Symbol:ATF4  
NCBI Official Synonym Symbols:CREB2; TXREB; CREB-2; TAXREB67  
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element-binding protein 67
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-4
UniProt Synonym Protein Names:Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67
Protein Family:Cyclic AMP-dependent transcription factor
UniProt Gene Name:ATF4  
UniProt Entry Name:ATF4_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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