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Human ATF1 / Activating Transcription Factor 2 ELISA Kit

SKU:
HUFI02237
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P18846
Sensitivity:
0.938ng/ml
Range:
1.563-100ng/ml
ELISA Type:
Sandwich
Synonyms:
ATF-1
Reactivity:
Human
Research Area:
Epigenetics and Nuclear Signaling
€599
Frequently bought together:

Description

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Human ATF1/Activating Transcription Factor 2 ELISA Kit

The Human ATF1 (Activating Transcription Factor 2) ELISA Kit is specifically designed for the quantitative measurement of ATF1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring precise and reproducible results for various research applications.ATF1 is a key transcription factor that plays a critical role in cellular processes such as differentiation, proliferation, and apoptosis. Dysregulation of ATF1 has been associated with various diseases including cancer, neurodegenerative disorders, and inflammatory conditions.

Therefore, studying ATF1 levels can provide valuable insights into the underlying mechanisms of these diseases and aid in the development of potential therapeutic interventions.Overall, the Human ATF1 ELISA Kit is a reliable tool for researchers seeking to investigate the role of ATF1 in disease pathogenesis and progression. Its high performance and accuracy make it a valuable asset for advancing our understanding of ATF1 biology and its implications for human health.

Product Name:Human ATF1 / Activating Transcription Factor 2 ELISA Kit
Product Code:HUFI02237
Size:96 Assays
Alias:ATF-1
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human ATF-1 concentrations in serum plasma and other biological fluids.
Sensitivity:0.938ng/ml
Range:1.563-100ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human ATF-1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human ATF-1 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)90-10195
EDTA plasma(n=5)88-10296
UFH plasma(n=5)86-10294
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ATF-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-105%90-98%85-100%
EDTA plasma(n=5)91-100%86-99%82-98%
UFH plasma(n=5)80-94%83-95%88-96%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP18846
UniProt Protein Function:ATF-1: a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation. Plays an important role in the trans-activation of the MHC class II trans-activator (CIITA) promoter III in B cells.
UniProt Protein Details:Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; protein complex binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: cellular protein complex assembly; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of DNA replication; positive regulation of transcription from RNA polymerase II promoter; response to cobalt ion; response to organic cyclic substance; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]
UniProt Code:P18846
NCBI GenInfo Identifier:1168542
NCBI Gene ID:466
NCBI Accession:P18846.2
UniProt Secondary Accession:P18846,P25168, Q9H4A8, B4DRF9,
UniProt Related Accession:P18846,AAB25878
Molecular Weight:15,295 Da
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-1
NCBI Synonym Full Names:activating transcription factor 1
NCBI Official Symbol:ATF1
NCBI Official Synonym Symbols:TREB36; EWS-ATF1; FUS/ATF-1
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-1
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-1
UniProt Synonym Protein Names:Activating transcription factor 1; Protein TREB36
Protein Family:Alcohol O-acetyltransferase
UniProt Gene Name:ATF1
UniProt Entry Name:ATF1_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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