The Human AST (Aspartate Aminotransferase) CLIA Kit is specially designed to accurately measure levels of AST in human serum and plasma samples. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for various research applications.AST is an important enzyme involved in amino acid metabolism and energy production in the body. Elevated levels of AST in the blood can indicate liver damage, heart disease, or muscle injury, making it a valuable biomarker for diagnosing and monitoring these conditions.
By using the Human AST CLIA Kit, researchers can gain valuable insights into the health status of patients and better understand the underlying mechanisms of various diseases. This kit is a valuable tool for studying liver function, cardiovascular health, and muscle disorders, helping to advance research and improve patient care.
Product Name:
Human AST (Aspartate Aminotransferase) CLIA Kit
SKU:
HUES00366
Target:
Human AST (Aspartate Aminotransferase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AST. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AST, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AST. You can calculate the concentration of Human AST in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
101-117
84-96
Average (%)
104
107
91
1:4
Range (%)
96-111
97-114
99-112
Average (%)
104
105
106
1:8
Range (%)
88-101
88-100
103-119
Average (%)
95
95
109
1:16
Range (%)
94-104
104-115
87-99
Average (%)
99
109
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.43
413.64
1807.16
180.45
392.78
1699.69
Standard deviation
22.28
48.23
143.49
19.92
35.08
134.45
C V (%)
11.17
11.66
7.94
11.04
8.93
7.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AST concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AST in samples. No significant cross-reactivity or interference between Human AST and analogues was observed.
