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Human Aspartate Aminotransferase 2 (AST2) ELISA Kit

SKU:
HUDL00268
Product Type:
ELISA Kit
Sensitivity:
0.089ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
GOT2,MitAAT,KATIV,KAT4,FABP-1,Plasma membrane-associated fatty acid-binding protein,Glutamic-Oxaloacetic Transaminase 2,Mitochondrial,Kynurenine Aminotransferase IV
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Aspartate Aminotransferase 2 (AST2) ELISA Kit

The Human Aspartate Aminotransferase 2 (AST2) ELISA Kit is a cutting-edge tool designed for the precise measurement of AST2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications. Aspartate Aminotransferase 2 (AST2) is a vital enzyme involved in amino acid metabolism, particularly in the conversion of aspartate to oxaloacetate.

Changes in AST2 levels have been linked to various liver and heart conditions, making it a valuable biomarker for studying and diagnosing these diseases. The Human AST2 ELISA Kit provides researchers with a reliable method for assessing AST2 levels and unlocking new insights into the role of this enzyme in health and disease.

Product Name:Human Aspartate Aminotransferase 2 (AST2) ELISA Kit
Product Code:HUDL00268
Size:96 Assays
Target:AST2
Synonyms:GOT2, MitAAT, KATIV, KAT4, FABP-1, Plasma membrane-associated fatty acid-binding protein, Glutamic-Oxaloacetic Transaminase 2,Mitochondrial, Kynurenine Aminotransferase IV
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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