Human Asialoglycoprotein Receptor 2 (ASGR2) ELISA Kit
The Human Asialoglycoprotein Receptor 2 (ASGR2) ELISA Kit is a powerful tool for the accurate measurement of ASGR2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ASGR2 is a key protein involved in the recognition and clearance of circulating glycoproteins by hepatocytes, playing a critical role in liver function and metabolism.
Dysregulation of ASGR2 has been linked to various liver diseases, making it a valuable biomarker for studying liver health and developing potential treatments.With its advanced technology and ease of use, the Human ASGR2 ELISA Kit is a valuable asset for researchers seeking to further understand the role of ASGR2 in liver physiology and pathology. Trust AssayGenie for reliable and accurate results in your ASGR2 research.
Product Name:
Human Asialoglycoprotein Receptor 2 (ASGR2) ELISA Kit
Product Code:
HUDL00259
Size:
96 Assays
Target:
ASGR2
Synonyms:
CLEC4H2, Asialoglyco Protein Receptor 2, C-type lectin domain family 4 member H2, Hepatic lectin H2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
