Human AS3MT Recombinant Protein (RPPB1416)
- SKU:
- RPPB1416
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9HBK9
- Research Area:
- Enzymes
Description
| Product Name: | Human AS3MT Recombinant Protein |
| Product Code: | RPPB1416 |
| Size: | 20µg |
| Species: | Human |
| Target: | AS3MT |
| Synonyms: | Arsenite methyltransferase, Methylarsonite methyltransferase, S-adenosyl-L-methionine:arsenic(III) methyltransferase, AS3MT, CYT19, RP11-753C18.6. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | AS3MT protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.15M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAALRD AEIQKDVQTY YGQVLKRSAD LQTNGCVTTA RPVPKHIREA LQNVHEEVAL RYYGCGLVIP EHLENCWILD LGSGSGRDCY VLSQLVGEKG HVTGIDMTKG QVEVAEKYLD YHMEKYGFQA SNVTFIHGYI EKLGEAGIKN ESHDIVVSNCVINLVPDKQQ VLQEAYRVLK HGGELYFSDV YTSLELPEEI RTHKVLWGEC LGGALYWKEL AVLAQKIGFC PPRLVTANLI TIQNKELERV IGDCRFVSAT FRLFKHSKTG PTKRCQVIYN GGITGHEKEL MFDANFTFKE GEIVEVDEET AAILKNSRFA QDFLIRPIGE KLPTSGGCSA LELKDIITDP FKLAEESDSM KSRCVPDAAG GCCGTKKSC |
Arsenic Methyltransferase (AS3MT) catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may have a role in arsenic metabolism. AS3MT methylates arsenite to produce methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite which is also a substrate, is transformed into the much less toxic complex dimethylarsinate (cacodylate), Me2As(O)-OH.
AS3MT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 399 amino acids (1-375 a.a.) and having a molecular mass of 44.3kDa.AS3MT is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AS3MT: Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me- AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH. Belongs to the methyltransferase superfamily. |
| UniProt Protein Details: | Protein type:EC 2.1.1.137; Methyltransferase; Mitochondrial Chromosomal Location of Human Ortholog: 10q24.32 Cellular Component: mitochondrion; cytoplasm; cytosol Molecular Function:arsenite methyltransferase activity; methylarsonite methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity Biological Process: methylation; arsonoacetate metabolic process; toxin metabolic process |
| NCBI Summary: | AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9HBK9 |
| NCBI GenInfo Identifier: | 109389364 |
| NCBI Gene ID: | 57412 |
| NCBI Accession: | NP_065733.2 |
| UniProt Secondary Accession: | Q9HBK9,Q0VDK3, Q0VDK4, Q5PZ02, A6NP79, |
| UniProt Related Accession: | Q9HBK9 |
| Molecular Weight: | 31,098 Da |
| NCBI Full Name: | arsenite methyltransferase |
| NCBI Synonym Full Names: | arsenite methyltransferase |
| NCBI Official Symbol: | AS3MT�� |
| NCBI Official Synonym Symbols: | CYT19�� |
| NCBI Protein Information: | arsenite methyltransferase; methyltransferase cyt19; methylarsonite methyltransferase; arsenic (+3 oxidation state) methyltransferase; S-adenosylmethionine:arsenic (III) methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase |
| UniProt Protein Name: | Arsenite methyltransferase |
| UniProt Synonym Protein Names: | Methylarsonite methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase |
| Protein Family: | Arsenite methyltransferase |
| UniProt Gene Name: | AS3MT�� |
| UniProt Entry Name: | AS3MT_HUMAN |
