The Human ARO (Aromatase) ELISA Kit is specifically designed for the precise measurement of aromatase levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable tool for a variety of research endeavors. Aromatase is a key enzyme responsible for the biosynthesis of estrogens, playing a crucial role in hormone regulation and various physiological processes. Dysregulation of aromatase activity has been linked to a variety of health conditions, including hormone-dependent cancers and estrogen-related disorders.
By detecting and quantifying aromatase levels, researchers can gain insights into disease progression and potential therapeutic targets. Whether investigating hormone-related diseases or exploring estrogen metabolism, the Human ARO (Aromatase) ELISA Kit offers a reliable and effective solution for researchers seeking to advance their understanding of aromatase biology.
Product Name:
Human ARO (Aromatase) ELISA Kit
SKU:
HUES01485
Target:
Human ARO (Aromatase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ARO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ARO. You can calculate the concentration of Human ARO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
90-106
99-114
Average (%)
91
98
105
1:4
Range (%)
92-103
83-97
84-96
Average (%)
98
89
91
1:8
Range (%)
90-101
86-98
85-98
Average (%)
95
93
92
1:16
Range (%)
89-102
86-99
87-98
Average (%)
94
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.1
3.7
0.59
1.2
3.92
Standard deviation
0.03
0.04
0.19
0.04
0.06
0.21
C V (%)
5.45
3.64
5.14
6.78
5.0
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ARO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ARO in samples. No significant cross-reactivity or interference between Human ARO and analogues was observed.
