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Human ARL1 Recombinant Protein (RPPB2783)

SKU:
RPPB2783
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P40616
€469

Description

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Product Name:Human ARL1 Recombinant Protein
Product Code:RPPB2783
Size:20µg
Species:Human
Target:ARL1
Synonyms:ARFL1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The ARL1 solution (0.25mg/ml) containing 20mM Tris-HCl pH-8, 2mM DTT, 100mM NaCl and 40% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTSSEMA NSLGLPALKD RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q

ARL1 is part of the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally associated to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, control intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. ARL1 is a weak stimulator of PLD and CT in a phospholipid dependent manner. ARL1 is a GTP-binding protein that has low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. ARL1 is involved in the Golgi apparatus.

ARL1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.5 kDa. The ARL1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:ARL1: GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:

Protein type:G protein; G protein, monomeric; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: Golgi membrane; Golgi apparatus; cytoplasm; trans-Golgi network

Molecular Function:GTPase activity; protein domain specific binding; GTP binding; metal ion binding; enzyme activator activity

Biological Process: phospholipase D activation; toxin metabolic process; protein localization in Golgi apparatus; small GTPase mediated signal transduction; retrograde transport, endosome to Golgi; Golgi organization and biogenesis; Golgi vesicle transport

NCBI Summary:The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:P40616
NCBI GenInfo Identifier:728888
NCBI Gene ID:400
NCBI Accession:P40616.1
UniProt Related Accession:P40616
Molecular Weight:20kDa
NCBI Full Name:ADP-ribosylation factor-like protein 1
NCBI Synonym Full Names:ADP ribosylation factor like GTPase 1
NCBI Official Symbol:ARL1��
NCBI Official Synonym Symbols:ARFL1��
NCBI Protein Information:ADP-ribosylation factor-like protein 1
UniProt Protein Name:ADP-ribosylation factor-like protein 1
Protein Family:ARL14 effector protein
UniProt Gene Name:ARL1��
UniProt Entry Name:ARL1_HUMAN

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