Human ARHGDI alpha (Rho GDP Dissociation Inhibitor Alpha ) CLIA Kit
The Human ARHGDI Alpha (Rho GDP Dissociation Inhibitor Alpha) CLIA Kit is specially designed for the precise measurement of ARHGDI Alpha levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ARHGDI Alpha, also known as Rho GDP Dissociation Inhibitor Alpha, is a key protein involved in regulating Rho GTPase signaling pathways that are critical for cell migration, adhesion, and cytoskeletal dynamics.
Dysregulation of these pathways is implicated in various diseases, including cancer, cardiovascular disorders, and immune disorders, highlighting the importance of ARHGDI Alpha as a potential biomarker for studying and developing therapeutic interventions for these conditions. Discover the versatility and reliability of the Human ARHGDI Alpha CLIA Kit for your research needs and uncover new insights into the role of ARHGDI Alpha in health and disease.
Product Name:
Human ARHGDI alpha (Rho GDP Dissociation Inhibitor Alpha ) CLIA Kit
SKU:
HUES00357
Target:
Human ARHGDI alpha (Rho GDP Dissociation Inhibitor Alpha )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ARHGDIα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARHGDIα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARHGDIα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ARHGDIα. You can calculate the concentration of Human ARHGDIα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
95-109
97-111
Average (%)
109
102
105
1:4
Range (%)
90-106
99-115
86-99
Average (%)
97
107
93
1:8
Range (%)
86-97
97-110
98-113
Average (%)
91
104
106
1:16
Range (%)
100-116
96-112
101-117
Average (%)
109
102
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
108
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.47
293.06
765.78
108.88
279.17
800.98
Standard deviation
8.87
26.05
64.78
13.41
31.74
80.74
C V (%)
8.41
8.89
8.46
12.32
11.37
10.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ARHGDIα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ARHGDIα in samples. No significant cross-reactivity or interference between Human ARHGDIα and analogues was observed.
