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Human Arginyl tRNA Synthetase (RARS) ELISA Kit

SKU:
HUDL02505
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P54136
Sensitivity:
0.30ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
ArgRS,DALRD1,Arginine tRNA Ligase 1,Cytoplasmic
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Arginyl tRNA Synthetase (RARS) ELISA Kit

The Human Arginyl-tRNA Synthetase (RARS) ELISA Kit is a highly reliable and sensitive assay designed for the precise measurement of RARS levels in human samples including serum, plasma, and cell culture supernatants. This kit offers unparalleled accuracy and reproducibility, making it an ideal tool for a wide range of research applications.RARS is a critical enzyme involved in protein synthesis, specifically in the activation of tRNA molecules with arginine.

Dysregulation of RARS has been linked to various diseases including neurological disorders and cancer, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.With its high sensitivity and specificity, the Human Arginyl-tRNA Synthetase (RARS) ELISA Kit provides researchers with a valuable tool for studying the role of RARS in disease pathogenesis and for exploring potential treatment strategies.

Product Name:Human Arginyl tRNA Synthetase (RARS) ELISA Kit
Product Code:HUDL02505
Size:96 Assays
Target:RARS
Synonyms:ArgRS, DALRD1, Arginine tRNA Ligase 1,Cytoplasmic
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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