Human Aquaporin 1, Colton Blood Group (AQP1) ELISA Kit
SKU:
HUDL00228
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29972
Sensitivity:
0.09ng/mL
Range:
0.25-16ng/mL
ELISA Type:
Sandwich
Synonyms:
AQP-CHIP,CHIP28,CO,Channel-Forming Integral Protein,28kDa,Aquaporin-CHIP,Urine water channel,Water channel protein for red blood cells and kidney proximal tubule
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Human Aquaporin 1, Colton Blood Group (AQP1) ELISA Kit
The Human Aquaporin-1 (AQP1) ELISA Kit is specifically designed for the precise and sensitive detection of AQP1 levels in human serum, plasma, and cell culture supernatants. This kit provides researchers with a reliable and accurate tool for studying the function and expression of AQP1, a key protein involved in water transport across cell membranes.Aquaporin-1 is a fundamental component in regulating water balance in cells and tissues, playing a crucial role in various physiological processes such as kidney function, fluid homeostasis, and cell migration.
Dysregulation of AQP1 has been implicated in numerous diseases including cancer, kidney disorders, and neurological conditions, underscoring its importance as a potential therapeutic target and diagnostic marker.With its exceptional sensitivity and specificity, the Human Aquaporin-1 (AQP1) ELISA Kit offers researchers a valuable resource for investigating the role of AQP1 in health and disease, paving the way for advancements in personalized medicine and targeted therapies.
Product Name:
Human Aquaporin 1, Colton Blood Group (AQP1) ELISA Kit
Product Code:
HUDL00228
Size:
96 Assays
Target:
AQP1
Synonyms:
AQP-CHIP, CHIP28, CO, Channel-Forming Integral Protein,28kDa, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule
Detection Method:
Sandwich
Range:
0.25-16ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
