Human AQP3 / Aquaporin-3 ELISA Kit
- SKU:
- HUFI00733
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q92482
- Sensitivity:
- 0.188ng/ml
- Range:
- 0.313-20ng/ml
- ELISA Type:
- Sandwich
- Synonyms:
- AQP-3, Aquaporin-3, AQP3, GIL, Aquaglyceroporin-3
- Reactivity:
- Human
- Research Area:
- Signal Transduction
Description
Human AQP3 / Aquaporin-3 ELISA
AQP3 / Aquaporin-3 is an integral membrane protein localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 / Aquaporin-3 can be functionally heterogenous and has been found to facilitate the transport of nonionic small solutes such as urea and glycerol. Mutations in AQP3 / Aquaporin-3 have been associated with Hereditary Papulotranslucent Acrokeratoderma. The Assay Genie Human AQP3/Aquaporin-3 ELISA is a highly sensitive assay for the quantitative measurement of AQP3/Aquaporin-3 in serum, blood, plasma, cell culture supernatant and tissue samples.
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human AQP3 / Aquaporin-3 ELISA Kit |
| Product Code: | HUFI00733 |
| Size: | 96 Assays |
| Alias: | AQP-3, Aquaporin-3, AQP3, GIL, Aquaglyceroporin-3 |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human AQP-3 concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.188ng/ml |
| Range: | 0.313-20ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery: | Matrices listed below were spiked with certain level of Human AQP-3 and the recovery rates were calculated by comparing the measured value to the expected amount of Human AQP-3 in samples.
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human AQP-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%): | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protein Information
| Uniprot: | |
| UniProt Protein Function: | AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. |
| UniProt Code: | |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | |
| NCBI Accession: | |
| UniProt Related Accession: | |
| Molecular Weight: | 29,987 Da |
| NCBI Full Name: | Aquaporin-3 |
| NCBI Official Symbol: | AQP3 |
| NCBI Official Synonym Symbols: | GIL; AQP-3 |
| NCBI Protein Information: | aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group) |
| UniProt Protein Name: | Aquaporin-3 |
Protocol
*Note: Protocols are specific to each batch/lot. For the exact instructions please follow the protocol included in your kit.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Procedure |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Aliquot 0.1ml standard solutions into the standard wells. |
| 3. | Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well. |
| 4. | Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells. |
| 5. | Seal the plate with a cover and incubate at 37 °C for 90 min. |
| 6. | Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2. |
| 7. | Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall. |
| 8. | Seal the plate with a cover and incubate at 37°C for 60 min. |
| 9. | Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash. |
| 10. | Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min. |
| 11. | Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min. |
| 12. | Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color. |
| 13. | Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately. |
| 14. | Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution. |
Sample Type
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
AQP3 Background
Aquaporin-3 (AQP3) is a protein belonging to the aquaporin family, which plays a vital role in facilitating the movement of water and other small molecules across cell membranes. AQP3 is predominantly found in the plasma membrane of tissues such as the skin, kidneys, and gastrointestinal tract. It functions as a water channel, aiding in the maintenance of water balance and homeostasis in the body. Additionally, AQP3 is involved in various biological processes and has implications in both health and disease.
AQP3 in Water Transport and Homeostasis
AQP3 acts as a water channel, allowing the movement of water molecules across cell membranes in response to osmotic gradients. Its presence in the kidneys enables the reabsorption of water, playing a crucial role in maintaining water balance and preventing excessive fluid loss through urine. In the skin, AQP3 facilitates the movement of water through the epidermis, contributing to skin hydration and barrier function. By participating in these water transport processes, AQP3 ensures the proper functioning of vital organs and tissues.
AQP3 in Cell Function and Solute Transport
Apart from its role in water transport, AQP3 has been associated with other cellular functions. It has been implicated in cell migration and proliferation, suggesting its involvement in processes like wound healing and tissue regeneration. AQP3 is also known to transport small solutes, such as glycerol and urea. This additional function expands its significance beyond water regulation and highlights its role in the transport of essential molecules required for cellular metabolism.
AQP3 in Tissue-specific Expression and Regulation
AQP3 is expressed in various tissues throughout the body, but its levels can vary. For example, AQP3 expression is notably high in the basolateral membranes of the collecting ducts in the kidneys, where water reabsorption takes place. In the skin, AQP3 is primarily found in the basal layer of the epidermis, contributing to water movement and skin hydration. The expression of AQP3 can be regulated by factors like hormones, cytokines, and environmental stimuli, allowing the body to adapt to changing water balance requirements and external conditions.
AQP3 FAQs
What is the AQP3 ELISA Kit?
The Human AQP3 ELISA Kit is a laboratory tool designed for the quantitative measurement of human Aquaporin-3 protein levels in biological samples.
What are the advantages of using the AQP3 ELISA Kit?
The Human AQP3 ELISA Kit offers a highly specific and sensitive method for quantifying AQP3 levels, enabling precise measurements in a range of sample types. Additionally, the kit is user-friendly, providing a streamlined protocol that can be easily implemented in the laboratory.
Where can I find more information about the AQP3 ELISA Kit?
For more detailed information about the AQP3 ELISA Kit, including technical specifications, performance characteristics, and ordering details, please refer to the product brochure or contact our customer support team. We are here to assist you with any inquiries you may have.
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