The Human AQP-3 (Aquaporin-3) ELISA Kit is a reliable and accurate tool for the quantitative measurement of AQP-3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity to ensure precise and reproducible results, making it suitable for various research applications.Aquaporin-3 is a key protein in the aquaporin family that plays a crucial role in regulating water transport in various tissues, including the skin, kidney, and gastrointestinal tract.
Dysregulation of AQP-3 has been linked to various skin disorders, such as psoriasis and atopic dermatitis, as well as conditions like cancer and inflammatory diseases.By using the Human AQP-3 ELISA Kit, researchers can gain valuable insights into the role of AQP-3 in health and disease, paving the way for the development of targeted therapies and diagnostic tools. This kit offers a convenient and efficient solution for studying the functions and dynamics of AQP-3 in different biological systems.
Product Name:
Human AQP-3 (Aquaporin 3) ELISA Kit
SKU:
HUES01693
Target:
Human AQP-3 (Aquaporin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AQP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AQP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AQP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AQP-3. You can calculate the concentration of Human AQP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
86-99
93-106
Average (%)
97
92
99
1:4
Range (%)
89-101
84-95
85-100
Average (%)
96
89
91
1:8
Range (%)
90-103
82-96
82-95
Average (%)
95
89
89
1:16
Range (%)
91-104
83-95
86-101
Average (%)
98
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
85-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.77
7.89
1.12
2.59
7.86
Standard deviation
0.06
0.14
0.38
0.06
0.15
0.32
C V (%)
5.66
5.05
4.82
5.36
5.79
4.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AQP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AQP-3 in samples. No significant cross-reactivity or interference between Human AQP-3 and analogues was observed.
