Human Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit
The Human Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit is specifically designed for the precise measurement of APAF1 levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it an invaluable tool for various research endeavors.APAF1 is a critical factor involved in the regulation of apoptotic pathways, playing a key role in programmed cell death. Dysregulation of APAF1 expression has been linked to various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
By accurately quantifying APAF1 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human APAF1 ELISA Kit provides researchers with a robust and reliable method for studying the role of APAF1 in health and disease, paving the way for innovative discoveries and potential therapeutic interventions.
Product Name:
Human Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit
Product Code:
HUDL00200
Size:
96 Assays
Target:
APAF1
Synonyms:
CED4, Apoptotic Protease Activating Factor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
