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Human Apolipoprotein C4 (APOC4) ELISA Kit

SKU:
HUDL00216
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P55056
Sensitivity:
37pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
Apo-C4,Apolipoprotein C-IV
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
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Description

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Human Apolipoprotein C4 (APOC4) ELISA Kit

The Human Apolipoprotein C4 (APOC4) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of APOC4 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it suitable for various research applications.Apolipoprotein C4 is a key player in lipid metabolism and is involved in regulating cholesterol levels in the body. Elevated levels of APOC4 have been associated with cardiovascular diseases and metabolic disorders, making it a valuable biomarker for studying these conditions and evaluating potential treatment options.

With its high sensitivity and specificity, the Human APOC4 ELISA Kit offers researchers a reliable tool for investigating the role of APOC4 in health and disease, ultimately advancing our understanding of lipid metabolism and its impact on human health.

Product Name:Human Apolipoprotein C4 (APOC4) ELISA Kit
Product Code:HUDL00216
Size:96 Assays
Target:APOC4
Synonyms:Apo-C4, Apolipoprotein C-IV
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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