The Human APOE (Apolipoprotein E) ELISA Kit is a highly sensitive and specific assay kit designed for the quantitative measurement of APOE levels in human samples such as serum, plasma, and cell culture supernatants. This kit is essential for researchers studying conditions related to lipid metabolism, cardiovascular diseases, and Alzheimer's disease, where APOE has been identified as a key biomarker.APOE is a crucial protein involved in lipid transport and metabolism, playing a critical role in regulating cholesterol levels in the blood.
Variations in the APOE gene have been linked to an increased risk of developing heart disease and Alzheimer's disease, making accurate measurement of APOE levels essential for understanding disease mechanisms and potential therapeutic strategies.With its high sensitivity and reproducibility, the Human APOE ELISA Kit from AssayGenie provides researchers with a reliable tool for studying the role of APOE in various disease processes and developing targeted treatments.
Product Name:
Human ApoE (Apolipoprotein E) ELISA Kit
SKU:
HUES01675
Target:
Human ApoE (Apolipoprotein E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
14.06 ng/mL
Detection range:
23.44-1500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoE. You can calculate the concentration of Human ApoE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
97-110
89-105
Average (%)
90
103
97
1:4
Range (%)
97-112
87-100
91-107
Average (%)
103
92
98
1:8
Range (%)
95-112
87-102
97-109
Average (%)
102
93
102
1:16
Range (%)
101-113
83-95
93-105
Average (%)
107
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
95-107
102
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
80.4
156.2
548.4
77.0
169.1
518.6
Standard deviation
5
7.3
25.8
5.2
7.8
28.0
C V (%)
6.22
4.67
4.7
6.75
4.61
5.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoE in samples. No significant cross-reactivity or interference between Human ApoE and analogues was observed.
