The Human ApoD (Apolipoprotein D) CLIA Kit is a cutting-edge technology designed for the precise measurement of Apolipoprotein D levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for a variety of research purposes.Apolipoprotein D is a key protein involved in lipid metabolism and is associated with various health conditions including neurodegenerative diseases, metabolic disorders, and cancer.
By measuring Apolipoprotein D levels, researchers can gain valuable insights into the pathogenesis of these diseases and develop potential therapeutic interventions.Trust in the Human ApoD CLIA Kit to provide reliable and reproducible data, enabling researchers to advance their understanding of Apolipoprotein D biology and its implications in health and disease. Order your kit today and take your research to the next level.
Product Name:
Human ApoD (Apolipoprotein D) CLIA Kit
SKU:
HUES00336
Target:
Human ApoD (Apolipoprotein D)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoD. You can calculate the concentration of Human ApoD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
101-116
96-113
Average (%)
100
109
103
1:4
Range (%)
99-115
91-104
96-111
Average (%)
106
97
102
1:8
Range (%)
92-106
89-102
90-105
Average (%)
97
95
96
1:16
Range (%)
87-101
100-117
85-100
Average (%)
95
107
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
96-113
104
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
368.17
1107.47
3181.52
349.56
1121.87
2866.73
Standard deviation
35.2
95.35
220.8
38.14
102.31
212.42
C V (%)
9.56
8.61
6.94
10.91
9.12
7.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoD in samples. No significant cross-reactivity or interference between Human ApoD and analogues was observed.
