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Human APOA5 Recombinant Protein (RPPB5718)

SKU:
RPPB5718
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q6Q788
Research Area:
Cytokines
$371
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Description

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Product Name:Human APOA5 Recombinant Protein
Product Code:RPPB5718
Size:10µg
Species:Human
Target:APOA5
Synonyms:Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3, APOA5, RAP3, APOAV.
Source:HEK293 Cells
Formulation:APOA5 Human was filtered (0.4�m) and lyophilized from 0.5 mg/ml supplied in 20mM TRIS and 50mM NaCl, pH 7.5
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
Stability:Store lyophilized APOA5 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted APOA5 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 70% as determined by SDS-PAGE.
Amino Acid Sequence:RKGFWDYFSQ TSGDKGRVEQ IHQQKMAREP ATLKDSLEQD LNNMNKFLEK LRPLSGSEAP RLPQDPVGMR RQLQEELEEV KARLQPYMAE AHELVGWNLE GLRQQLKPYT MDLMEQVALR VQELQEQLRV VGEDTKAQLL GGVDEAWALL QGLQSRVVHH TGRFKELFHP YAESLVSGIG RHVQELHRSV APHAPASPAR LSRCVQVLSR KLTLKAKALH ARIQQNLDQL REELSRAFAG TGTEEGAGPD PQMLSEEVRQ RLQAFRQDTY LQIAAFTRAI DQETEEVQQQ LAPPPPGHSA FAPEFQQTDS GKVLSKLQAR LDDLWEDITH SLHDQGHSHL GDPAAADYKD DDDK

ApoA5 is an important regulator of serum triglyceride concentrations. An ApoA5 mouse nock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. Furthermore, peroxisome proliferators-activated receptoragonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. There is very little known about ApoA5 protein in human serum. Lately, it was established that ApoA5 is present in the human serum and it can be detected by polyclonal antibodies against both the HN2 and COOH termini, but at much lower concentration than other apolipoproteins.

APOA5 Human Recombinant Flag-Tagged Fusion Protein is 40.1 kDa protein containing 354 amino acid residues of the APOA5 Human and 11 additional amino acid residues of flagTag.

UniProt Protein Function:APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: chylomicron; extracellular region; extracellular space

Molecular Function:cholesterol binding; cholesterol transporter activity; enzyme activator activity; enzyme binding; heparin binding; lipid binding; low-density lipoprotein receptor binding; phosphatidylcholine binding; phospholipid binding

Biological Process: acylglycerol homeostasis; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; lipid transport; lipoprotein metabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipid catabolic process; positive regulation of lipoprotein lipase activity; positive regulation of receptor-mediated endocytosis; regulation of cholesterol absorption; reverse cholesterol transport; tissue regeneration; triacylglycerol catabolic process; triacylglycerol metabolic process

Disease: Hyperlipoproteinemia, Type V; Hypertriglyceridemia, Familial

NCBI Summary:The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]
UniProt Code:Q6Q788
NCBI GenInfo Identifier:60391728
NCBI Gene ID:116519
NCBI Accession:Q6Q788.1
UniProt Secondary Accession:Q6Q788,Q3MIK6, Q6UWK9, Q9UBJ3, B0YIV9,
UniProt Related Accession:Q6Q788
Molecular Weight:41,213 Da
NCBI Full Name:Apolipoprotein A-V
NCBI Synonym Full Names:apolipoprotein A5
NCBI Official Symbol:APOA5��
NCBI Official Synonym Symbols:RAP3; APOAV��
NCBI Protein Information:apolipoprotein A-V
UniProt Protein Name:Apolipoprotein A-V
UniProt Synonym Protein Names:Apolipoprotein A5; Regeneration-associated protein 3
Protein Family:Apolipoprotein
UniProt Gene Name:APOA5��
UniProt Entry Name:APOA5_HUMAN

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