Human ApoA5 (Apolipoprotein A5) ELISA Kit (AEES00077)

Product Name:	Human ApoA5 (Apolipoprotein A5) ELISA Kit
SKU:	AEES00077
Target:	Human ApoA5 (Apolipoprotein A5)
Size:	96T
Assay type:	Sandwich-ELISA
Assay time:	3.5h
Sensitivity:	37.50 pg/mL
Detection range:	62.50-4000 pg/mL

Kit component:

Component	Quantity (24 Assays)	Quantity (96 Assays)	Storage
Micro ELISA Plate (Dismountable)	8 wells x 3 strips	8 wells x 12 strips	-20°C, 12 months
Reference Standard	1 vial	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 60 µL	1 vial, 120 µL
Concentrated HRP Conjugate (100×)	1 vial, 60 µL	1 vial, 120 µL	-20°C (shading light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	1 vial, 20 mL	4°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	1 vial, 10 mL	4°C (shading light)
Stop Solution	1 vial, 10 mL	1 vial, 10 mL	4°C
Plate Sealer	5 pieces	5 pieces
Product Description	1 copy	1 copy

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoA5. You can calculate the concentration of Human ApoA5 in the samples by comparing the OD of the samples to the standard curve.

Linearity:

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	83-95	97-106	84-97
1:2	Average (%)	90	102	91
1:4	Range (%)	87-101	101-107	90-102
1:4	Average (%)	93	104	95
1:8	Range (%)	97-108	86-97	102-113
1:8	Average (%)	105	93	105
1:16	Range (%)	84-96	87-96	91-101
1:16	Average (%)	90	90	95

Recovery:

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	89-103	96
EDTA plasma (n=5)	89-100	94
Cell culture media (n=5)	100-109	103

Precision:

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20.0	20.0	20.0	20.0	20.0
Mean (ng/mL)	168.97	447.94	1846.03	177.36	498.83	1844.5
Standard deviation	10.14	22.4	82.33	9.08	22.6	95.55
C V (%)	6	5.0	4.46	5.12	4.53	5.18

Sample type &Sample volume:	serum, plasma and other biological fluids; 100μL
Reproducibility:	Both intra-CV and inter-CV are < 10%.
Application:	This ELISA kit applies to the in vitro quantitative determination of Human ApoA5 concentrations in serum, plasma and other biological fluids.
Specificity:	This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed.