The Human APOA2 (Apolipoprotein A2) CLIA Kit is a highly sensitive and specific assay designed for the precise quantification of APOA2 levels in human biological samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing researchers with a reliable tool for investigating the role of APOA2 in various physiological and pathological processes.APOA2 is a key protein involved in lipid metabolism and is known to play a critical role in the regulation of cholesterol levels in the body.
Abnormalities in APOA2 expression have been linked to conditions such as cardiovascular disease, obesity, and metabolic disorders, highlighting its importance as a potential biomarker for assessing and monitoring these health conditions.With its high sensitivity and accuracy, the Human APOA2 CLIA Kit offers researchers a valuable tool for studying the impact of APOA2 on human health and disease, paving the way for the development of novel therapeutic strategies targeting APOA2-related pathways.
Product Name:
Human ApoA2 (Apolipoprotein A2) CLIA Kit
SKU:
HUES00329
Target:
Human ApoA2 (Apolipoprotein A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 ng/mL
Detection range:
12.50-800 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoA2. You can calculate the concentration of Human ApoA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
85-99
88-100
Average (%)
96
91
93
1:4
Range (%)
96-111
101-114
102-115
Average (%)
102
108
109
1:8
Range (%)
92-103
88-103
86-100
Average (%)
98
95
91
1:16
Range (%)
86-101
102-118
95-109
Average (%)
92
108
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
96-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.91
68.23
364.55
38.06
74.45
354.8
Standard deviation
5.24
4.84
41.56
4.44
8.74
23.95
C V (%)
12.81
7.09
11.4
11.67
11.74
6.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA2 in samples. No significant cross-reactivity or interference between Human ApoA2 and analogues was observed.
