Human APLN (Apelin) ELISA Kit (HUES01662)
- SKU:
- HUES01662
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q9ULZ1
- Sensitivity:
- 37.5pg/mL
- Range:
- 62.5-4000pg/mL
- ELISA Type:
- Competitive
- Synonyms:
- APEL, XNPEP2
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cardiovascular
Description
Product Name: | Human APLN (Apelin) ELISA Kit |
SKU: | HUES01662 |
Target: | Human APLN (Apelin) |
Size: | 96T |
Assay type: | Competitive-ELISA |
Assay time: | 2.0h |
Sensitivity: | 37.50 pg/mL |
Detection range: | 62.50-4000 pg/mL |
Kit component: |
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This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with APLN. During the reaction, APLN in the sample or standard competes with a fixed amount of APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum, plasma and other biological fluids; 50μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of APLN concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes APLN in samples. No significant cross-reactivity or interference between APLN and analogues was observed. |