Human APIP Recombinant Protein (RPPB2764)
- SKU:
- RPPB2764
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96GX9
Description
| Product Name: | Human APIP Recombinant Protein |
| Product Code: | RPPB2764 |
| Size: | 20µg |
| Species: | Human |
| Target: | APIP |
| Synonyms: | APAF1 interacting protein, CGI-29, APIP2, Mmrp19, APAF1-interacting protein, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase, CGI29, EC 4.2.1.109, dJ179L10.2, MMRP19. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The APIP solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV |
APIP catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). APIP has an anti-apoptotic function and inhibits muscle ischemic impairment. APIP inhibits the cytochrome c-dependent and APAF1-mediated cell death.
APIP Human Recombinant produced E. coli is a single polypeptide chain containing 266 amino acids (1-242) and having a molecular mass of 29.7kDa.APIP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MMRP19: Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Has an anti-apoptotic function and prevents muscle ischemic damage. Inhibits the cytochrome c- dependent and APAF1-mediated cell death. Belongs to the aldolase class II family. MtnB subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 4.2.1.109; Amino Acid Metabolism - cysteine and methionine; Apoptosis Chromosomal Location of Human Ortholog: 11p13 Cellular Component: cytoplasm Molecular Function:identical protein binding; 5-methylthioribulose-1-phosphate 4-dehydratase activity; protein binding; zinc ion binding Biological Process: methionine salvage; sulfur amino acid metabolic process; apoptosis; methionine biosynthetic process from S-adenosylmethionine; polyamine metabolic process; negative regulation of apoptosis |
| NCBI Summary: | APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008] |
| UniProt Code: | Q96GX9 |
| NCBI GenInfo Identifier: | 74731866 |
| NCBI Gene ID: | 51074 |
| NCBI Accession: | Q96GX9.1 |
| UniProt Secondary Accession: | Q96GX9,Q6PJX6, Q8WVU2, Q96HK2, Q9Y318, A8K9D3, |
| UniProt Related Accession: | Q96GX9 |
| Molecular Weight: | 242 |
| NCBI Full Name: | Methylthioribulose-1-phosphate dehydratase |
| NCBI Synonym Full Names: | APAF1 interacting protein |
| NCBI Official Symbol: | APIP�� |
| NCBI Official Synonym Symbols: | APIP2; CGI29; hAPIP; CGI-29; MMRP19�� |
| NCBI Protein Information: | methylthioribulose-1-phosphate dehydratase; APIP; MTRu-1-P dehydratase; APAF1-interacting protein; probable methylthioribulose-1-phosphate dehydratase |
| UniProt Protein Name: | Methylthioribulose-1-phosphate dehydratase |
| UniProt Synonym Protein Names: | APAF1-interacting protein |
| UniProt Gene Name: | APIP�� |
| UniProt Entry Name: | MTNB_HUMAN |
