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Human APEX Nuclease 1 (APEX1) ELISA Kit

SKU:
HUDL00202
Product Type:
ELISA Kit
Sensitivity:
0.132ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
APE,APE1,APEN,APEX,APX,HAP1,REF1,Multifunctional DNA Repair Enzyme,APEX Nuclease 1,Redox factor-1,DNA-(apurinic or apyrimidinic site) lyase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human APEX Nuclease 1 (APEX1) ELISA Kit

The Human APEX Nuclease 1 (APEX1) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of APEX1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides dependable and reproducible results, making it suitable for a variety of research applications.APEX1 is a critical enzyme involved in base excision repair and DNA damage response pathways, playing a key role in maintaining genomic stability and integrity.

Dysregulation of APEX1 has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Therefore, this ELISA kit is essential for studying APEX1's function in these conditions and potentially developing targeted therapies.Overall, the Human APEX Nuclease 1 ELISA Kit offers researchers a reliable tool for quantifying APEX1 levels and gaining valuable insights into its role in disease pathology.

Product Name:Human APEX Nuclease 1 (APEX1) ELISA Kit
Product Code:HUDL00202
Size:96 Assays
Target:APEX1
Synonyms:APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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