The Human ANXA1 (Annexin A1) ELISA Kit is specifically designed for the quantitative measurement of annexin A1 levels in human serum, plasma, and cell culture supernatants. This innovative kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. Annexin A1 is a key protein involved in the regulation of inflammation, cell signaling, and apoptosis. It plays a crucial role in various diseases, including inflammatory disorders, cancer, and autoimmune conditions.
As a vital biomarker, studying annexin A1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets. Don't miss out on the opportunity to advance your research with the Human ANXA1 ELISA Kit, a powerful tool for detecting and quantifying annexin A1 levels with precision and efficiency.
Product Name:
Human ANXA1 (Annexin A1) ELISA Kit
SKU:
HUES03491
Target:
Human ANXA1 (Annexin A1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANXA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANXA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANXA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANXA1. You can calculate the concentration of Human ANXA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
93-110
97-110
Average (%)
95
100
104
1:4
Range (%)
93-104
85-99
89-101
Average (%)
99
91
95
1:8
Range (%)
90-102
80-93
83-93
Average (%)
97
87
88
1:16
Range (%)
91-108
85-98
83-94
Average (%)
98
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
91-108
99
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.91
2.01
9.28
0.88
2.07
9.54
Standard deviation
0.06
0.09
0.47
0.05
0.1
0.51
C V (%)
6.59
4.48
5.06
5.68
4.83
5.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANXA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANXA1 in samples. No significant cross-reactivity or interference between Human ANXA1 and analogues was observed.
