null

Human Antigen Peptide Transporter 1 (TAP1) ELISA Kit

SKU:
HUDL02827
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q03518
Sensitivity:
0.134ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
APT1,ABC17,ABCB2,PSF1,RING4,Y3,TAP1N,Peptide Supply Factor 1,Atp-Binding Cassette,Subfamily B,Member 2,Peptide transporter involved in antigen processing 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Antigen Peptide Transporter 1 (TAP1) ELISA Kit

The Human Antigen Peptide Transporter-1 (TAP1) ELISA Kit is a cutting-edge tool for accurately measuring TAP1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a wide range of research applications in immunology, oncology, and infectious diseases.TAP1 is a key protein involved in the antigen processing and presentation pathway, playing a crucial role in the immune response against pathogens and cancer cells.

Abnormal TAP1 levels have been linked to various autoimmune diseases, viral infections, and tumor progression, making it a valuable biomarker for understanding and treating these conditions.With the Human Antigen Peptide Transporter-1 (TAP1) ELISA Kit, researchers can confidently study the role of TAP1 in immune surveillance, inflammation, and disease progression, leading to potential breakthroughs in diagnostics and therapeutics.

Product Name:Human Antigen Peptide Transporter 1 (TAP1) ELISA Kit
Product Code:HUDL02827
Size:96 Assays
Target:TAP1
Synonyms:APT1, ABC17, ABCB2, PSF1, RING4, Y3, TAP1N, Peptide Supply Factor 1, Atp-Binding Cassette,Subfamily B,Member 2, Peptide transporter involved in antigen processing 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products