Human Anti-Sperm IgG Autoantibodies ELISA Kit (HDES0077)
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- SKU:
- HDES0077
- Size:
- 96T
- Reactivity:
- Human
- Detection Method:
- Indirect ELISA
- Target:
- AS-Ab
- Detection Wavelength:
- 450/630nm
Description
| Product Name: | Human Anti-Sperm IgG Autoantibodies ELISA Kit |
| SKU: | HDES0077 |
| Size: | 96T |
| Target: | AS-Ab |
| Detection Method: | Indirect ELISA |
| Reactivity: | Human |
| Result Type: | Qualitative: Sensitivity 98.1%, Specificity 99.9% |
| Sample Type: | Serum |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the method to detect the Sperm (SPE) antibody (IgG) in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with purified SPE membrane antigen. Samples are added to the ELISA Microtiter plate wells and the SPE antibody in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugate is added to each well and react with the compound to form “SPE antigen- SPE antibody-HRP conjugate” compound. The TMB substrate is added to initiate the color developing reaction. The presence of SPE antibody (IgG) can be determined according to the OD value after colorimetric assay with the Microplate reader.
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