Description
Product Name: | Human Islet Cell (IC) Autoantibodies ELISA Kit |
SKU: | HDES0033 |
Size: | 96T |
Target: | IC-Ab |
Detection Method: | Indirect ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.3%, Specificity 99.4% |
Sample Type: | Serum |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the principle to detect the Islet Cell (IC) antibody in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with purified IC antigen. Samples are added to the ELISA Microtiter plate wells and the IC-Ab in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugate is added to each well and react with the compound to form “IC antigen- IC antibody-HRP conjugate” compound. The substrate reagent is added to initiate the color developing reaction. The presence of IC-Ab can be determined according to the OD value after colorimetric assay with the Micro-plate reader.