Description
Product Name: | Human Anti-Cardiolipin (ACA) Autoantibodies ELISA Kit |
SKU: | HDES0075 |
Size: | 96T |
Target: | ACA-Ab |
Detection Method: | Indirect ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.5%, Specificity 99.8% |
Sample Type: | Serum |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the method to detect the ACA-Ab in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with purified ACA antigen. Samples are added to the ELISA Microtiter plate wells and the ACA-Ab in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugated Mouse-anti-human IgG antibody is added to each well and react with the compound to form “ACA antigen- ACA antibody-HRP antibody” compound. The TMB substrate is added to initiate the color developing reaction. The presence of ACA-Ab can be determined according to the OD value after colorimetric assay with the micro-plate reader.