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Human Anthrax Toxin Receptor 2 (ANTXR2) ELISA Kit

SKU:
HUDL00183
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P58335
Sensitivity:
0.133ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CMG-2,CMG2,ISH,JHF,Capillary Morphogenesis Protein 2
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Anthrax Toxin Receptor 2 (ANTXR2) ELISA Kit

The Human Anthrax Toxin Receptor 2 (ANTXR2) ELISA Kit is a powerful tool for the precise measurement of ANTXR2 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it perfect for various research applications.ANTXR2 is a critical receptor protein involved in mediating the entry of anthrax toxin into the cells, playing a key role in anthrax infection.

By detecting ANTXR2 levels, researchers can gain valuable insights into the mechanisms of anthrax toxin uptake and potential therapeutic interventions. This ELISA kit is an essential resource for studying anthrax infection and developing strategies for effective prevention and treatment.

Product Name:Human Anthrax Toxin Receptor 2 (ANTXR2) ELISA Kit
Product Code:HUDL00183
Size:96 Assays
Target:ANTXR2
Synonyms:CMG-2, CMG2, ISH, JHF, Capillary Morphogenesis Protein 2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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