The Human ANP (Atrial Natriuretic Peptide) ELISA Kit is specifically designed for the precise detection of atrial natriuretic peptide levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it perfect for a variety of research purposes.Atrial natriuretic peptide is a vital hormone involved in regulating blood pressure and fluid balance by promoting vasodilation and excretion of sodium and water.
It plays a crucial role in conditions like heart failure, hypertension, and renal disorders, making it a key biomarker for understanding these diseases and developing potential treatments. Overall, the Human ANP ELISA Kit provides researchers with a valuable tool for studying the role of atrial natriuretic peptide in various physiological and pathological processes, paving the way for advancements in disease diagnosis and management.
Product Name:
Human ANP (Atrial Natriuretic Peptide) ELISA Kit
SKU:
HUES01727
Target:
Human ANP (Atrial Natriuretic Peptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ANP. During the reaction, ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ANP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
92-108
94-108
Average (%)
103
99
99
1:4
Range (%)
90-105
85-100
95-106
Average (%)
96
92
100
1:8
Range (%)
85-96
91-104
94-106
Average (%)
90
97
100
1:16
Range (%)
90-104
92-103
95-108
Average (%)
96
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
94-104
99
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.8
74.5
197.1
29.0
80.3
208.6
Standard deviation
1.7
3.9
8.9
1.7
4.6
11.5
C V (%)
6.34
5.23
4.52
5.86
5.73
5.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ANP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed.
