Human ANGPTL3 (Angiopoietin Like Protein 3) CLIA Kit
The Human ANGPTL3 (Angiopoietin-like protein 3) CLIA Kit is a reliable tool for quantifying levels of ANGPTL3 in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an invaluable resource for various research applications.ANGPTL3 is a key player in lipid metabolism and has been implicated in various metabolic disorders including cardiovascular diseases and dyslipidemia.
By accurately measuring ANGPTL3 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets.In summary, the Human ANGPTL3 CLIA Kit offers researchers a powerful tool for studying the role of ANGPTL3 in human health and disease, paving the way for advancements in personalized medicine and targeted therapies.
Product Name:
Human ANGPTL3 (Angiopoietin Like Protein 3) CLIA Kit
SKU:
HUES00238
Target:
Human ANGPTL3 (Angiopoietin Like Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANGPTL3. You can calculate the concentration of Human ANGPTL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
87-99
92-103
Average (%)
91
93
98
1:4
Range (%)
87-100
102-115
100-113
Average (%)
92
108
107
1:8
Range (%)
85-96
94-106
96-111
Average (%)
90
99
103
1:16
Range (%)
92-108
95-110
92-106
Average (%)
100
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.97
189.42
758.89
98.73
196.08
793.85
Standard deviation
11.37
13.64
86.06
8.12
21.39
85.5
C V (%)
11.73
7.2
11.34
8.22
10.91
10.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANGPTL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL3 in samples. No significant cross-reactivity or interference between Human ANGPTL3 and analogues was observed.
