Human ANGPTL2 (Angiopoietin Like Protein 2) ELISA Kit
The Human ANGPTL2 (Angiopoietin Like Protein 2) ELISA Kit is meticulously crafted for the quantitative assessment of human ANGPTL2 levels across a variety of biological samples.
ANGPTL2, as a secreted glycoprotein, is involved in multiple physiological and pathological processes, including inflammation, angiogenesis, and metabolism regulation. Understanding ANGPTL2 expression levels is crucial for investigating its diverse roles in cardiovascular diseases, metabolic disorders, and cancer. Accurate measurement of ANGPTL2 is essential for elucidating its intricate functions in health and disease. With exceptional sensitivity and specificity, our ANGPTL2 ELISA Kit delivers reliable and reproducible results, enabling precise quantification for research and clinical applications. Manufactured under strict quality control measures, this kit ensures robust performance and user-friendly protocols, making it an invaluable tool for studying the roles of ANGPTL2 in various biological contexts. Trust Assay Genie's ANGPTL2 ELISA Kit for dependable and accurate quantification of this critical protein in your research endeavors.
Product Name:
Human ANGPTL2 (Angiopoietin Like Protein 2) ELISA Kit
SKU:
AEES00070
Target:
Human ANGPTL2 (Angiopoietin Like Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANGPTL2. You can calculate the concentration of Human ANGPTL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
95-106
90-98
Average (%)
91
98
95
1:4
Range (%)
92-101
87-101
88-99
Average (%)
95
95
91
1:8
Range (%)
98-107
98-111
86-98
Average (%)
102
103
93
1:16
Range (%)
94-103
87-98
94-110
Average (%)
100
95
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
89-98
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.07
4.66
0.46
1.24
4.82
Standard deviation
0.03
0.05
0.25
0.03
0.06
0.26
C V (%)
6.12
4.67
5.36
6.52
4.84
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANGPTL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL2 in samples. No significant cross-reactivity or interference between Human ANGPTL2 and analogues was observed.
