The Human ANGPT4 (Angiopoietin 4) CLIA Kit is specifically designed for the precise measurement of Angiopoietin 4 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Angiopoietin 4 is a key protein involved in the regulation of angiogenesis, which is the formation of new blood vessels. Its role in angiogenesis makes it a crucial factor in various health conditions, including cancer, cardiovascular diseases, and neurodegenerative disorders.
By detecting and measuring Angiopoietin 4 levels, researchers can gain valuable insights into these conditions and potentially develop innovative treatments.Overall, the Human ANGPT4 CLIA Kit provides researchers with a powerful tool to study the role of Angiopoietin 4 in angiogenesis and its implications for various diseases. Its reliable performance and precise measurements make it an essential asset for cutting-edge research in the field of molecular biology and medicine.
Product Name:
Human ANGPT4 (Angiopoietin 4) CLIA Kit
SKU:
HUES00235
Target:
Human ANGPT4 (Angiopoietin 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPT4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPT4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANGPT4. You can calculate the concentration of Human ANGPT4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
88-102
96-113
Average (%)
92
93
103
1:4
Range (%)
95-108
92-108
85-100
Average (%)
100
99
92
1:8
Range (%)
100-117
96-113
95-107
Average (%)
108
104
101
1:16
Range (%)
96-109
87-101
87-100
Average (%)
104
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
104
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
100-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.86
300.31
973.57
99.81
291.76
1051.18
Standard deviation
11.51
28.5
62.6
11.26
23.31
87.88
C V (%)
11.19
9.49
6.43
11.28
7.99
8.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANGPT4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPT4 in samples. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed.
