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Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

SKU:
HUDL00112
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P30556
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AT1R,AG2S,AG2-S,AGTR1A,AGTR1B,AT1,AT1B,AT2R1,AT2-R1,AT2R1A,AT2R1B,HAT1R,AT1 Receptor,Angiotensin Receptor 1B
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

The Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit is specifically designed for the accurate detection of AGTR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.AGTR1 is a key receptor involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance.

Dysregulation of AGTR1 has been linked to various cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.With its advanced technology and reliable performance, the Human AGTR1 ELISA Kit is a valuable tool for researchers looking to explore the role of AGTR1 in health and disease.

Product Name:Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit
Product Code:HUDL00112
Size:96 Assays
Target:AGTR1
Synonyms:AT1R, AG2S, AG2-S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2-R1, AT2R1A, AT2R1B, HAT1R, AT1 Receptor, Angiotensin Receptor 1B
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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