Human Angiogenesis Array (43 targets) (SARB0018)
- SKU:
- SARB0018
- Product Type:
- Protein Array
- Analytes:
- Angiogenin
- Angiopoietin-1
- Angiopoietin-2
- Angiostatin
- bFGF
- EGF
- ENA-78 (CXCL5)
- Endostatin
- GCSF
- GM-CSF
- GRO alpha/beta/gamma
- I-309 (TCA-3/CCL1)
- IFN-gamma
- IGF-1
- IL-10
- IL-1 alpha (IL-1 F1)
- IL-1 beta (IL-1 F2)
- IL-2
- IL-4
- IL-6
- IL-8 (CXCL8)
- I-TAC (CXCL11)
- Leptin
- MCP-1 (CCL2)
- MCP-3 (MARC/CCL7)
- MCP-4 (CCL13)
- MMP-1
- MMP-9
- PDGF-BB
- PECAM-1 (CD31)
- PLGF
- RANTES (CCL5)
- TGF beta 1
- Thrombopoietin (TPO)
- Tie-2
- TIMP-1
- TIMP-2
- TNF alpha
- uPAR
- VEGF-A
- VEGFR2
- VEGFR3
- VEGF-D
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
| Product Sku: | SARB0018 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 43 |
| Gene Symbols: | ANG, ANGPT1, ANGPT2, CCL1, CCL13, CCL2, CCL5, CCL7, COL18A1, CSF2|CSF3, CXCL1, CXCL11, CXCL2, CXCL3, CXCL5, CXCL8, EGF, FGF2, FIGF, FLT4, IFNG, IGF1, IL10, IL1A, IL1B, IL2, IL4, IL6, KDR, LEP, MMP1, MMP9, PDGFB, PECAM1, PGF, PLAUR, PLG, TEK, TGFB1, THPO, TIMP1, TIMP2, TNF, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| Angiopoietin-1
| Angiopoietin-2
| Angiostatin
| bFGF
|
EGF
| ENA-78 (CXCL5)
| Endostatin
| GCSF
| GM-CSF
|
GRO alpha/beta/gamma
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
| IL-10
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-4
| IL-6
|
IL-8 (CXCL8)
| I-TAC (CXCL11)
| Leptin
| MCP-1 (CCL2)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| MMP-1
| MMP-9
| PDGF-BB
| PECAM-1 (CD31)
|
PLGF
| RANTES (CCL5)
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-2
|
TIMP-1
| TIMP-2
| TNF alpha
| uPAR
| VEGF-A
|
VEGFR2
| VEGFR3
| VEGF-D
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Angiogenesis Antibody Array C1000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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