Human Angiogenesis Array (23 targets)

Product Sku:	SARB0020
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	23
Gene Symbols:	ANGPT1, ANGPT2, CCL1, CCL13, CCL7, COL18A1, CSF2, CSF3, CXCL11, FLT4\|IL10, IL1A, IL1B, IL2, IL4, KDR, MMP1, MMP9, PECAM1, PLAUR, PLG, TEK, TNF
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123	Angiostatin Gene Symbol: PLG Gene ID: 5340 Accession #: P00747	Endostatin Gene Symbol: COL18A1 Gene ID: 80781 Accession #: P39060	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919
GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584
IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098	MCP-4 (CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616
MMP-1 Gene Symbol: MMP1 Gene ID: 4312 Accession #: P03956	MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780	PECAM-1 (CD31) Gene Symbol: PECAM1 Gene ID: 5175 Accession #: P16284	Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375
uPAR Gene Symbol: PLAUR Gene ID: 5329 Accession #: Q03405	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis