The Human ANG2 (Angiopoietin-2) CLIA Kit is specifically designed for the precise measurement of Angiopoietin-2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and accuracy, ensuring reliable and consistent results for a variety of research applications.Angiopoietin-2 is a key regulator of angiogenesis, playing a crucial role in blood vessel development and function.
Dysregulation of Angiopoietin-2 has been implicated in various diseases including cancer, cardiovascular disorders, and inflammatory conditions. As such, this CLIA kit is an essential tool for studying the role of Angiopoietin-2 in these diseases and for the development of potential therapeutic interventions.Order now to unlock the potential of Angiopoietin-2 research with the Human ANG2 CLIA Kit from Assay Genie.
Product Name:
Human ANG2 (Angiopoietin 2) CLIA Kit
SKU:
HUES00008
Target:
Human ANG2 (Angiopoietin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANG2. You can calculate the concentration of Human ANG2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
86-98
86-98
Average (%)
102
93
92
1:4
Range (%)
99-113
101-115
98-112
Average (%)
107
107
105
1:8
Range (%)
86-101
100-114
94-106
Average (%)
93
108
99
1:16
Range (%)
103-118
100-113
92-106
Average (%)
109
107
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.85
120.5
444.77
52.82
130.9
459.26
Standard deviation
6.07
12.41
40.52
6.64
11.57
42.07
C V (%)
11.94
10.3
9.11
12.57
8.84
9.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANG2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG2 in samples. No significant cross-reactivity or interference between Human ANG2 and analogues was observed.
