Human ANG-R-Tie2 (Angiopoietin Receptor Tie2) CLIA Kit
The Human ANG-R (TIE2 Angiopoietin Receptor TIE2) CLIA Kit is specifically designed for the quantitative measurement of ANG-R levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.TIE2 is a critical receptor involved in the regulation of angiogenesis, playing a key role in vascular development and maintenance. Dysregulation of TIE2 signaling has been implicated in various diseases, including cancer and cardiovascular disorders.
The Human ANG-R CLIA Kit enables researchers to study the role of TIE2 in disease pathogenesis and to evaluate its potential as a therapeutic target.Overall, the Human ANG-R CLIA Kit is a valuable tool for investigating the molecular mechanisms underlying angiogenesis and vascular biology, offering insights into the development of novel therapies for angiogenesis-related diseases.
Product Name:
Human ANG-R-Tie2 (Angiopoietin Receptor Tie2) CLIA Kit
SKU:
HUES00242
Target:
Human ANG-R-Tie2 (Angiopoietin Receptor Tie2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG-R-Tie2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG-R-Tie2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG-R-Tie2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANG-R-Tie2. You can calculate the concentration of Human ANG-R-Tie2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-119
102-115
98-115
Average (%)
110
108
106
1:4
Range (%)
92-109
96-112
89-103
Average (%)
99
104
94
1:8
Range (%)
88-100
100-115
89-106
Average (%)
95
107
97
1:16
Range (%)
101-117
89-100
99-112
Average (%)
108
95
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
95-110
100
Cell culture media (n=5)
98-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.19
173.46
707.33
98.04
184.37
723.77
Standard deviation
8.46
18.98
61.68
8.67
17.37
80.63
C V (%)
8.2
10.94
8.72
8.84
9.42
11.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANG-R-Tie2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG-R-Tie2 in samples. No significant cross-reactivity or interference between Human ANG-R-Tie2 and analogues was observed.
