The Human ANG (Angiogenin) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of angiogenin levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent choice for a variety of research applications.Angiogenin is a key protein involved in angiogenesis, the process of forming new blood vessels. Its role in promoting blood vessel formation and influencing cell proliferation makes it a crucial biomarker for studying conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders.
The Human ANG CLIA Kit provides researchers with a valuable tool for investigating these conditions and exploring potential therapeutic interventions. Overall, this kit offers a valuable resource for researchers looking to study angiogenin levels in human samples and gain insights into its role in various diseases and biological processes. Visit the provided URL for more information on the Human ANG (Angiogenin) CLIA Kit.
Product Name:
Human ANG (Angiogenin) CLIA Kit
SKU:
HUES00006
Target:
Human ANG (Angiogenin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANG. You can calculate the concentration of Human ANG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
98-110
98-111
Average (%)
101
104
105
1:4
Range (%)
103-115
100-114
87-100
Average (%)
109
107
93
1:8
Range (%)
95-109
101-120
96-114
Average (%)
101
109
104
1:16
Range (%)
88-102
93-108
89-103
Average (%)
93
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
103
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
95-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.95
252.88
924.5
113.6
240.62
994.18
Standard deviation
9.51
23.52
83.02
10.41
23.58
81.03
C V (%)
8.81
9.3
8.98
9.16
9.8
8.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG in samples. No significant cross-reactivity or interference between Human ANG and analogues was observed.
