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Human and Mouse Phospho-MLKL (S345) PharmaGenie ELISA Kit (SBRS1900)

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SKU:
SBRS1900
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human and Mouse Phospho-MLKL S345 PharmaGenie ELISA Kit SBRS1900
  • Human and Mouse Phospho-MLKL S345 PharmaGenie ELISA Kit SBRS1900
€1,349

Description

Human and Mouse Phospho-MLKL (S345) PharmaGenie ELISA Kit

Product SKU:SBRS1900
Size:96T
Application:The antibody pair provided in this kit recognizes Human and Mouse MLKL phosphorylated at site Serine-345.
Uniprot:Q8NB16
Gene ID:197259
Pathway:Necroptosis
Synonyms:Mixed lineage kinase domain-like protein (hMLKL)
Target Species:Human, Mouse
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human and Mouse Phospho-MLKL (S345) PharmaGenie ELISA Kit (SBRS1900) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageHT29 cells were treated with Calyculin A. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageHT29 cells were treated with Calyculin A. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

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