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Human Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit

SKU:
HUDL00163
Product Type:
ELISA Kit
Sensitivity:
0.64ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
AMY2A,AMY2B,PA,1,4-alpha-D-glucan glucanohydrolase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit

The Human Alpha-2 Pancreatic Amylase (AMY2) ELISA Kit is a powerful tool for the precise measurement of AMY2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts outstanding sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.AMY2 is a key enzyme responsible for breaking down complex carbohydrates in the digestive system.

Its levels in the body can provide valuable insights into pancreatic health and function, as well as aid in the diagnosis and monitoring of conditions such as pancreatic disorders and diabetes. By utilizing this ELISA kit, researchers can uncover vital information about AMY2 activity and its implications in various diseases, paving the way for innovative treatments and therapies.

Product Name:Human Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit
Product Code:HUDL00163
Size:96 Assays
Target:AMY2
Synonyms:AMY2A, AMY2B, PA, 1,4-alpha-D-glucan glucanohydrolase
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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