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Human Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) ELISA Kit

SKU:
HUDL02617
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51168
Sensitivity:
0.107ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ENaCb,ENaCbeta,SCNEB,Liddle Syndrome,Sodium Channel,Nonvoltage-Gated 1,Beta,Epithelial Na(+) channel subunit beta,Nonvoltage-gated sodium channel 1 subunit beta
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) ELISA Kit

The Human Amiloride-Sensitive Sodium Channel Subunit Beta (SCNN1B) ELISA Kit is a reliable and accurate tool for detecting levels of SCNN1B in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.SCNN1B is a crucial subunit of the amiloride-sensitive sodium channel, playing a vital role in ion transport and fluid balance regulation in the body. Dysregulation of SCNN1B has been associated with conditions like hypertension, cystic fibrosis, and pulmonary diseases.

Therefore, this ELISA kit is invaluable for studying the role of SCNN1B in these conditions and developing potential therapies.Overall, the Human SCNN1B ELISA Kit is a valuable asset for researchers looking to explore the function and significance of SCNN1B in various physiological and pathological processes. Its accuracy, sensitivity, and ease of use make it a versatile tool for laboratories and research facilities.

Product Name:Human Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) ELISA Kit
Product Code:HUDL02617
Size:96 Assays
Target:SCNN1b
Synonyms:ENaCb, ENaCbeta, SCNEB, Liddle Syndrome, Sodium Channel,Nonvoltage-Gated 1,Beta, Epithelial Na(+) channel subunit beta, Nonvoltage-gated sodium channel 1 subunit beta
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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