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Human AMD1 Recombinant Protein (RPPB1399)

SKU:
RPPB1399
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P17707
Research Area:
Enzymes
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Description

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Product Name:Human AMD1 Recombinant Protein
Product Code:RPPB1399
Size:5µg
Species:Human
Target:AMD1
Synonyms:Adenosylmethionine decarboxylase 1, S-adenosylmethionine decarboxylase proenzyme, AdoMetDC, S-adenosylmethionine decarboxylase 1, SAMDC, AMD, EC 4.1.1.50.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The AMD1 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80% as determined by SDS-PAGE.
Amino Acid Sequence:GSSHHHHHH SSGLVPRGSH MGSHMSSMFV SKRRFILKTC GTTLLLKALV PLLKLARDYS GFDSIQSFFY SRKNFMKPSH QGYPHRNFQE EIEFLNAIFP NGAAYCMGRM NSDCWYLYTL DFPESRVISQ PDQTLEILMS ELDPAVMDQF YMKDGVTAKD VTRESGIRDL IPGSVIDATM FNPCGYSMNG MKSDGTYWTI HITPEPEFSY VSFETNLSQT SYDDLIRKVV EVFKPGKFVT TLFVNQSSKC RTVLASPQKI EGFKRLDCQS AMFNDYNFVF TSFAKKQQQQ QS

Adenosylmethionine decarboxylase proenzyme (AMD1) is synthesized originally as an inactive proenzyme. Putrescine stimulates both the proenzyme processing and the catalytic activity. The catalytic activity is inhibited by iodoacetic acid. The active enzyme formation entails a self-maturation process in which the active site pyruvoyl group is produced from an internal serine residue using an autocatalytic post-translational modification.

AMD1 Human Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (68-334) and having a molecular mass of 33.4 kDa.AMD1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:AMD1: an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Two alternatively spliced transcript variants that encode different proteins have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y.[provided by RefSeq, Jul 2010]
UniProt Protein Details:

Protein type:Amino Acid Metabolism - arginine and proline; Lyase; Amino Acid Metabolism - cysteine and methionine; EC 4.1.1.50

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytosol

Molecular Function:adenosylmethionine decarboxylase activity

Biological Process: S-adenosylmethioninamine biosynthetic process; spermidine biosynthetic process; spermine biosynthetic process; polyamine metabolic process

NCBI Summary:This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Multiple alternatively spliced transcript variants have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y. [provided by RefSeq, Dec 2013]
UniProt Code:P17707
NCBI GenInfo Identifier:116241324
NCBI Gene ID:262
NCBI Accession:P17707.2
UniProt Secondary Accession:P17707,Q5VXN4, Q5VXN6, Q9BWK4, E1P5F7,
UniProt Related Accession:P17707
Molecular Weight:21,301 Da
NCBI Full Name:S-adenosylmethionine decarboxylase proenzyme
NCBI Synonym Full Names:adenosylmethionine decarboxylase 1
NCBI Official Symbol:AMD1��
NCBI Official Synonym Symbols:AMD; SAMDC; ADOMETDC��
NCBI Protein Information:S-adenosylmethionine decarboxylase proenzyme; S-adenosylmethionine decarboxylase 1
UniProt Protein Name:S-adenosylmethionine decarboxylase proenzyme
Protein Family:S-adenosylmethionine decarboxylase proenzyme
UniProt Gene Name:AMD1��
UniProt Entry Name:DCAM_HUMAN

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