Human ALPL (Alkaline Phosphatase,Liver/Bone/Kidney) ELISA Kit
The Human ALPL (Alkaline Phosphatase Liver, Bone, Kidney) ELISA Kit is specifically designed for the precise measurement of ALPL levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results, making it an excellent choice for various research applications.ALPL is an important enzyme involved in bone mineralization and various metabolic processes in the liver and kidneys.
Dysregulation of ALPL levels is associated with several diseases and conditions, including bone disorders, liver disease, and kidney dysfunction. The Human ALPL ELISA Kit provides researchers with a valuable tool for studying these conditions and exploring potential therapeutic interventions.Get accurate and reliable results with the Human ALPL ELISA Kit from Assay Genie. Order yours today and advance your research in the field of bone, liver, and kidney health.
Product Name:
Human ALPL (Alkaline Phosphatase,Liver/Bone/Kidney) ELISA Kit
SKU:
HUES03390
Target:
Human ALPL (Alkaline Phosphatase,Liver/Bone/Kidney)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ALPL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALPL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALPL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ALPL. You can calculate the concentration of Human ALPL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
93-105
91-105
Average (%)
95
99
97
1:4
Range (%)
88-102
83-93
85-98
Average (%)
94
89
91
1:8
Range (%)
88-102
85-97
85-98
Average (%)
93
92
92
1:16
Range (%)
92-107
80-90
87-102
Average (%)
99
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
232.41
686.93
2152.29
235.06
692.44
2121.0
Standard deviation
15.27
28.64
98.79
13.26
38.85
75.51
C V (%)
6.57
4.17
4.59
5.64
5.61
3.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ALPL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ALPL in samples. No significant cross-reactivity or interference between Human ALPL and analogues was observed.
