Human alphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit (HUES03283)
- SKU:
- HUES03283
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P01189(138~150aa)
- Sensitivity:
- 0.09ng/mL
- Range:
- 0.16-10ng/mL
- ELISA Type:
- Competitive
- Synonyms:
- alpha-melanotropin, alpha-melanocortin, alpha-intermedin
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
Description
Product Name: | Human alphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit |
SKU: | HUES03283 |
Target: | Human alphaMSH (Alpha-Melanocyte Stimulating Hormone) |
Size: | 96T |
Assay type: | Competitive-ELISA |
Assay time: | 2.0h |
Sensitivity: | 0.10 ng/mL |
Detection range: | 0.16-10 ng/mL |
Kit component: |
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This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with αMSH. During the reaction, αMSH in the sample or standard competes with a fixed amount of αMSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to αMSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of αMSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum, plasma and other biological fluids; 50μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of αMSH concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes αMSH in samples. No significant cross-reactivity or interference between αMSH and analogues was observed. |