Human alphaHSP (Alpha-Hemoglobin Stabilizing Protein) CLIA Kit
The Human AlphaHsp (Alpha Hemoglobin Stabilizing Protein) CLIA Kit is specifically designed for the precise measurement of alpha hemoglobin stabilizing protein levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for a variety of research purposes.Alpha Hemoglobin Stabilizing Protein is a critical regulator of hemoglobin stability, function, and transport, playing a crucial role in the maintenance of hemoglobin levels and oxygen delivery in the body.
Dysregulation of this protein has been linked to various hematological disorders and conditions, making it a valuable biomarker for investigating these diseases and potential therapeutic interventions.Overall, the Human AlphaHsp CLIA Kit provides researchers with a reliable tool for studying the role of alpha hemoglobin stabilizing protein in health and disease, offering valuable insights into potential diagnostic and treatment strategies for related disorders.
Product Name:
Human alphaHSP (Alpha-Hemoglobin Stabilizing Protein) CLIA Kit
SKU:
HUES00870
Target:
Human alphaHSP (Alpha-Hemoglobin Stabilizing Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human αHSP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human αHSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human αHSP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human αHSP. You can calculate the concentration of Human αHSP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-112
103-116
89-101
Average (%)
102
109
95
1:4
Range (%)
96-110
95-108
90-106
Average (%)
101
101
97
1:8
Range (%)
102-114
91-105
100-118
Average (%)
108
98
108
1:16
Range (%)
88-103
98-115
87-101
Average (%)
95
105
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
104
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.92
266.79
753.97
101.99
245.02
785.17
Standard deviation
11.56
29.35
56.77
8.5
27.74
68.23
C V (%)
10.52
11.0
7.53
8.33
11.32
8.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human αHSP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human αHSP in samples. No significant cross-reactivity or interference between Human αHSP and analogues was observed.
