A1-ACT,SERPINA3,AACT,ACT,GIG24,GIG25,Serpin Peptidase Inhibitor Clade A Member 3(Alpha-1 Antiproteinase,Antitrypsin),Cell growth-inhibiting gene 24/25 protein
The Human Alpha-1-Antichymotrypsin (A1ACT) ELISA Kit is a cutting-edge tool for precise measurement of alpha-1-antichymotrypsin levels in human samples including serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Alpha-1-antichymotrypsin is a vital inhibitor of proteinases that plays a crucial role in inflammatory responses and regulation of proteolytic activities.
Dysregulation of alpha-1-antichymotrypsin has been linked to various diseases, making it a valuable biomarker for studying conditions such as Alzheimer's disease, rheumatoid arthritis, and cancer.Enhance your research capabilities with the Human Alpha-1-Antichymotrypsin ELISA Kit, a reliable tool for exploring the role of alpha-1-antichymotrypsin in health and disease. Order yours today and unlock the potential for groundbreaking discoveries in your scientific investigations.
Product Name:
Human Alpha-1-Antichymotrypsin (a1ACT) ELISA Kit
Product Code:
HUDL00001
Size:
96 Assays
Target:
a1ACT
Synonyms:
A1-ACT, SERPINA3, AACT, ACT, GIG24, GIG25, Serpin Peptidase Inhibitor Clade A Member 3(Alpha-1 Antiproteinase, Antitrypsin), Cell growth-inhibiting gene 24/25 protein
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
