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Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) ELISA Kit

SKU:
HUDL00151
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10696
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ALPG,GCAP,ALP-1,Alkaline phosphatase Nagao isozyme,Germ cell alkaline phosphatase,Placental alkaline phosphatase-like
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) ELISA Kit

The Human Alkaline Phosphatase Placental-Like Protein 2 (ALPPL2) ELISA Kit is a powerful tool for quantifying ALPPL2 levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it ideal for a variety of research applications.ALPPL2 is an important protein involved in cellular processes and has been linked to various diseases and conditions.

Its role in cancer, cardiovascular diseases, and neurodegenerative disorders makes it a valuable biomarker for understanding these conditions and potentially developing new therapeutic strategies.Designed to meet the needs of researchers and clinicians alike, the Human ALPPL2 ELISA Kit offers a reliable and efficient solution for studying the functions and implications of ALPPL2 in human health and disease.

Product Name:Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) ELISA Kit
Product Code:HUDL00151
Size:96 Assays
Target:ALPPL2
Synonyms:ALPG, GCAP, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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